FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-7_S51_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-7_S51_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC100.7949125596184419No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG90.7154213036565978Illumina Single End PCR Primer 1 (100% over 50bp)
AGAATAACGATATTAAAACCTTCAAGAAAGATGACATCCAACAAGTCAAC60.47694753577106513No Hit
CTTTAATCAGTTGAATACATGGTGGTAGGTATTCAATGTGGATGTTTTTA60.47694753577106513No Hit
GTTGCCTTTCTCTTTCGAACTTTGGAATTGACTTTGAAGCCACATTAGAC50.39745627980922094No Hit
CGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTCTAG40.3179650238473768No Hit
CACCGATGCTGGCAGCAACTTTGTCGATGCCGCAAAGGCCCTTGGATTGA40.3179650238473768No Hit
CTTTCTCACAAAATTTCATAAACGGAGTCAAAGACGGAATGAAAGACCTA40.3179650238473768No Hit
GAAAGTCGCGCTCTAGTAAAAAAGTCCACAAACTTATCGTACAATTTCAT40.3179650238473768No Hit
CTGGCTCGTCAGGAGAGTCAATAAGACATTGGACACCAAGGCCAAGCGAA40.3179650238473768No Hit
AAGTAACAGACTTTATATGGAACCATATTAAATATTTTCTGTTGGCATTC40.3179650238473768No Hit
TATTATTCGAGATGTTAATAGAGGGTGAGTGATTCGTAATTGCCATGTTA40.3179650238473768No Hit
TTCGGTTTTTTAAAAAGCGCAGTGCTTTATGGGGCTTGGTGGTTACAGTA40.3179650238473768No Hit
CTGAAAGACACGTTATTTTGAATGCATTTTACAACAGCATTGTAGGAAAG40.3179650238473768No Hit
TGAACATAGTGGCAGGGTCAAGGCTGCCGCTGATGCTAATACTGACGTTA40.3179650238473768No Hit
AATTCGTACCGTTTCATATTAAGGACATATTTCAAAATGGGAGGAGGAGA40.3179650238473768No Hit
AAAAGGGCAAGGAGCTGAGAAAGGCAGACGACATCAAGGAAGTGGAGAAA40.3179650238473768No Hit
CATAACTCCAAAAGTAGTAAATGGAATCCAACAACACTTGGAACATGGAT30.23847376788553257No Hit
GGTCAAGTGACCAATAAAGCATATTCTCGTTTTCATTGTTGTATATTATG30.23847376788553257No Hit
CCGCTTTCCAGACTATCTCTGCTACCCACAGGGAATCTCTGAACAAACTC30.23847376788553257No Hit
TTTTTTTCTTCTAGTGGAACCTGTTATTCTTCTGTATTTATGTGATGTTT30.23847376788553257No Hit
TTTCACTCGACTTGCTGTATTTGAAGACTGTACAAGAATAATTCACTCTT30.23847376788553257No Hit
CGCCCATCTACTCATTTGACGGCAAGAACGTCAAGGAAGATCCAAAATGG30.23847376788553257No Hit
CTTAACTGAAATTAGCGAAAAGAACGCTTCTTCTGTAGTGTTACTATTAT30.23847376788553257No Hit
TGCATGTTACCCTTTACAAATGAGTACAGATGTGTTTATTAACTTCAAAT30.23847376788553257No Hit
GCTTAACCAGCTCCAGTGTAATGGTGTACTTGAAGGTATCCGTATCTGTA30.23847376788553257No Hit
AATTTTACTTCAAACCTCTGTATCTTTAATTCAAGATCATTTTTCGTGAT30.23847376788553257No Hit
TTGCCACCACCTCCTTCCTTCTTTTTTGCGTGTGTTCTCATTTTTATTTT30.23847376788553257No Hit
TTTTAATTGAAGAAAGGAATGAAAGGTTTGACGTAATGGTGACTGTCTCA30.23847376788553257No Hit
CTTTTCTACGCTACGAAATTCAATTCAGTTAAAGATAGTTTCTCATGTTG30.23847376788553257No Hit
GGCCACCGCTGCCTCCTCATCTTCTCTTGAGAAGAGCTACGAACTCCCCG30.23847376788553257No Hit
TAAAAAACCCGTAAATTTATCCCCCATATGTAAAATGCAATAAGCAAAAG30.23847376788553257No Hit
CAAAAAATAAAAATGTCATAATACTACCCTGTTTTGTTTTCGATATCAAA30.23847376788553257No Hit
CTTCTTTCACATATCGGTAGTCTTGAAATTGAAGAAACATCGTACAAGGA30.23847376788553257No Hit
AATTCAGCATCGCAACTGATCGTCGAGACAAGCTACAGCGAGCTACCAAA30.23847376788553257No Hit
GGACAGACACATTTGTCAAATATCACAGCTATGGCGTCGTCACCAACATG30.23847376788553257No Hit
AAATCTTCTTCTCATAAACGGTCCAACAGATTTTCAAAATGTTTATGTAG30.23847376788553257No Hit
GGATGGTAAATTCTCTCAGGTTATGTCCAACGGTCTTGATAACAAGCTCC30.23847376788553257No Hit
CGTCTTCAGACACAGGTCTTCAAGCTTGGTTTGGCCAAGAGTATCCATCA30.23847376788553257No Hit
GCGGGCCGACCAGTTACTCCACCGAGAGGGACAGCCAACAAAGGCAAAGG30.23847376788553257No Hit
GCCTGTAGGCGTATTGTGTTTTATTACAGGGGGATATTGTTTATATTTAT30.23847376788553257No Hit
CCCTTCTTTAAATTTTGTGAGGGAATGTTTTTTGGTTTGTAGGATTTTAT30.23847376788553257No Hit
GCGACATTAAAATGTCTTATATTGAACAAAAACTTGTTTTTATGCAGATA30.23847376788553257No Hit
TTTGAAGGAAGGGGCAATTTTTTTTAATGTTCTTAGGAGTTAATTTAACG30.23847376788553257No Hit
GAAAGAGATGTCTGGAACGTTTGATGGTAAAAAAGCATGCTGGGTACCAC30.23847376788553257No Hit
GAAGAAGTTGGCCATCAGATATGTCCTGGACTGTTCTCATCCAGTAGAAG30.23847376788553257No Hit
GGAACCCATGCTGATGAGCAAACCTGTCACAGCTAAAGGGGACTTCACGT30.23847376788553257No Hit
GTTCCAGATAGTGCTTGATGTGTTCATGTTTAAGCATTAAAACTTTGCTT30.23847376788553257No Hit
AGGAGAACTAAGATGGCTCAAACCTAACGGATCTGGACAAAATAGTTCGA30.23847376788553257No Hit
TGTGGATGTCCAGTTCGATGATAATTTACCACCTATGTTAAATGCTTTGG30.23847376788553257No Hit
GGAATCTGCAGGGTTTAGGGTCTGGATTGGAATATGCTGGGATAGGGTCC30.23847376788553257No Hit
GTGTAAACCTGTTTAATTGACATCACAAAATATGTTGCAATTTAAAGCGG30.23847376788553257No Hit
ACAGCTCTGAAGGATTCATAATTTGCTAATTAATCATTCATAAATCACAG30.23847376788553257No Hit
GAAGTCATGTCTCCTGGATTGATAGTGCTACCAACTGTCAGCTAATCGGG30.23847376788553257No Hit
TCAGCACCAGTCATCAAATGTAGGGAACCAGTCCTCCCAGCCGATTGGAT30.23847376788553257No Hit
AGGTGATATATAGATAAGGAACTCGGCAAAACTACTTTTCGCTTGTTTAA30.23847376788553257No Hit
GTCAAACAATCCTTTGGGAGTCATAAGTCATGCAGATAAAGTCCCTTTTC30.23847376788553257No Hit
CTAAAATAAGAATACTGTATAAACTGTGCATTTTCAAAAGTCCTGAAATT20.1589825119236884No Hit
GGGGAAGAATCTTTTTTATAGGGGTAATAGTAATTTTATGATTCTTTAAT20.1589825119236884No Hit
AATGAGTTTGTTAAAGAATACCAATCTTTTTTTTTTTTTTTTCTTTTAGT20.1589825119236884No Hit
CATGCATGTAATAAATATATACTGTGGTCAAGAATGATTGTAGATATCGA20.1589825119236884No Hit
GTTCATTTGACTAATCATGTGACTATTCATGTGACACCACGAGGCAGGAC20.1589825119236884No Hit
GTTAAATCGGCATTATATTGAGCATACACAAATAGATGTATGGATAACTA20.1589825119236884No Hit
GAGTAGGACTGTTCGTCCTTTAAAATTCTACATGATCTGAGTTCAGACCG20.1589825119236884No Hit
TGACAATCTTTCATTTGCAAATGGTGCAAAATTTTCCACAAAAGACCAGG20.1589825119236884No Hit
GCGGACAGAACAAACAATTTTCAGCAGCTAAAAACACGGCAAAACTGGAC20.1589825119236884No Hit
GCTCTATGCCAGCTACGTGTACTTGTCTATGGCCTACTACTTCGACCGTG20.1589825119236884No Hit
ATAACCTTTATGAACTGGTGTGCTATAAACCCTCATTTCACTGCCACAAT20.1589825119236884No Hit
CTACTGACAAGTCCATCACTCCTCTTGGTGGCTTCCCTCATTACGGAGAA20.1589825119236884No Hit
AGAGACTGTTCTTCAAGATCACGTCTCAATTTCAACAATTCTTGTTCACG20.1589825119236884No Hit
ATTTTGAGTTTGGAATATTTTATTGATCCTCAAATCAAACTTAAGAAATA20.1589825119236884No Hit
TTGTTATTAAGGAACTTGACGATCTGGGACTCTCAAACAACACGATTATT20.1589825119236884No Hit
CACAAACACATAACACACAAAAATGCAGCATACATGCACAAACTCACACA20.1589825119236884No Hit
GCTACATCTTAATTTGAAATCAATTTAATACATTATCATGGTACAAAAAG20.1589825119236884No Hit
ATAGAGACTTGTTTTGTGTAATGTATACATGTGATACAGGACTTTGCGTA20.1589825119236884No Hit
CTTTCTTCCAGGCTCTGCAGATTACAACTAAGATCACGAAGGGATGCATT20.1589825119236884No Hit
CTCCATTTAAGACAAAGTCATACATCCGACGGGTCCTTGTCCAAACGTTT20.1589825119236884No Hit
GAACTGTTTAATAATAATGATAGTAGTCTATGATCACAATTACTTTTGTG20.1589825119236884No Hit
GAGGTATAAATGGTGTACATCCCCGACCTAGAAAAATTATGCAGCTGTTC20.1589825119236884No Hit
AGATGAAGCTGTTGCTTATGGTGCAGCTGTACAGGCAGCCATTCTGCATG20.1589825119236884No Hit
ACGTCACACTTGACAAGTTTGCAAAGTTATCTAAGTCAGGCATTAACTCA20.1589825119236884No Hit
CGAAAATATCAATACACAAAATAAAAACATAATTGACAGAATTAAGTGTT20.1589825119236884No Hit
GTCATCACATATTTACGGTTGGCATAAATATTGATAGGCGAGCTTATTTT20.1589825119236884No Hit
CAAACATAATCATAATGTATTTATATGATAGACACTTCATAAAGTCATGT20.1589825119236884No Hit
TATACTGCATATCTATGTTCACATTAGAAGCTTTGTTTATGTATACCATT20.1589825119236884No Hit
TAACACTGAAATGGTAGCTTATGTATAATATTCTTATTTGTATCTTCTAT20.1589825119236884No Hit
TCTGCCATTGCCTATGACTGGTTCCCTTTATATGAAATGTAATAAATAGT20.1589825119236884No Hit
TCTATTTTGGGGGCTATTAATTTCATTTCTACAACTGTGAATATACGGCC20.1589825119236884No Hit
TGGCAAGGAACTGAACAAAAGTATTAACCCAGATGAAGCTGTTGCTTATG20.1589825119236884No Hit
CGACAACAGACATGAGAGTTGACCTTCACCTTTGAACATTGCTACCATAC20.1589825119236884No Hit
GTCAAAGTTCTCATCTACAGCAGTAAACTTGTGAAGATCTGATATAATAT20.1589825119236884No Hit
AGTAATACCAACAACAGTAACAACAACAGCAACACTATCAGCAACAACAA20.1589825119236884No Hit
GTTTGGGGTTGTTTTGTTTTAGAGTTTAAAATTTTAAAAACTTTTCATAT20.1589825119236884No Hit
TGGATTCCAATGCGAAATGTTGTATTTATATTTTCGAATTTTCGATGTAA20.1589825119236884No Hit
TGACTTCTCACTGCTGGGGTTGAGGAGTTGAGCCTCTGCCAGTCACTTGC20.1589825119236884No Hit
GAGACAGAATTGTTCGAGCTTTCCTTATTGAAGAACAGAAAATTGTTGTC20.1589825119236884No Hit
TATGATGAAGAAAAAAAAAATTGGTTCTTCAAAATGAAATGCGAAAACTT20.1589825119236884No Hit
CATAACTCTATTCTTGCTAATTACAGAGTTATTGCCCTTTGATATATTTC20.1589825119236884No Hit
CAGAGCAGCATCCATCTGCAGACATTTGTACATCAGATCACCAGCTGGAG20.1589825119236884No Hit
ATACAAAAAAAATAATAAAATATGTTTAAAGTTAGTATGGTCCGGATTTT20.1589825119236884No Hit
TTTGGTTTCAGAGATAAAAAAAAGTACAACTGATTGCATTTTCATATTAC20.1589825119236884No Hit
TTGTCATGCCGAACCTGATGAAGTTGATCATGTTGGACACTAACATGGTA20.1589825119236884No Hit
CGAAAACGAGTTCGACAAAGTCAACGAACAATTCCAGGAGGGTGTCGTGA20.1589825119236884No Hit
AGAGGCCGCACTCAGAAGGGTAAGATACATGTACGGTAACACACACACTG20.1589825119236884No Hit
CGTGACTTGACTCAGAGACTTAAGGACGCCGAGAACCGTGCTGCTGAGGC20.1589825119236884No Hit
GTGAAGCCCTCAAATCATGTACACAGAGCGCTGAGTGTTCAGCATGTAGC20.1589825119236884No Hit
GTCCAACGTTTCAACACTGATTCTGCAATATAATGAACTGAAGACCTTGC20.1589825119236884No Hit
GCCGCATCCGTGACTACGCCCATAGAAAGATGGTACACATCTTAAGGAGG20.1589825119236884No Hit
ACTTGTTGCGCGAGTTGATGCGGGAGACTACTTTGTGATTGGACCGAACT20.1589825119236884No Hit
AAAAAATGTATAATTATATGCCAATGTGTTAACAATTCCATTGCTGTAAC20.1589825119236884No Hit
CTTAGACAGTTTTACTTTTTGTATCATGGTTTATAGAGTGTAAAAAATTA20.1589825119236884No Hit
GTCTTGTATTCTCGATATTTCATCATGTTCGTTGGTGATGGTCTGTTGAA20.1589825119236884No Hit
CCTATGACTGCAGAGGATAGATTCTTGCGAAAACAATGGCTCAGAGACCA20.1589825119236884No Hit
CAAAAATGCACTTTTGACTGCAATGTATTTCTTTTATGGTGCAGATTATA20.1589825119236884No Hit
GTCAGAGACATCAAGGAGAAGCTCTGCTATGTTGCCCTGGACTTCGAGCA20.1589825119236884No Hit
GGTGACTCCAAAAACGATCCTCCAAAGGCAGCAACCAATTTCAATGCCCA20.1589825119236884No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.1589825119236884No Hit
CTCAGATTCATTCTTAGATCTTCCTCCACCATTAAAACTGGACATGCCAT20.1589825119236884No Hit
CAGAATGGCGTGGTCAGGTATGGCCGGCAGGATTCCACAATGCTGATGAG20.1589825119236884No Hit
CGGCAATTTACGTGCGAGAGCAGAGAAACACTGTGCTATGAATCGGAAGT20.1589825119236884No Hit
CTGGACTAAGAAATCACTGGTTATCCCAGATTCATGCTGCAAGACGCCAT20.1589825119236884No Hit
CGCCATCCTCCGTCTTGACTTGGCTGGCAGAGATCTTACAGATTACTTGA20.1589825119236884No Hit
GTTCGTAGGACCAAGTATTAGTGTTCCGGTCCAACTTTTTATAGTTTTTT20.1589825119236884No Hit
GTTTCAAAGGCTCAATCTCAAGGGCAGAGGAGAAATGGAAATTGTTTGAC20.1589825119236884No Hit
GGAAATCACCGCTCTTGCTCCATCCACCATGAAGATCAAGATCATTGCTC20.1589825119236884No Hit
CCGAGTACTGGTGAACCTTTCAGGATTCCAGGAAAGATTGGTTAGATTAG20.1589825119236884No Hit
CCCGATTTGAGGAAATCAACAAAGAAGTTTCCACCTACATCAAAAAGATT20.1589825119236884No Hit
TCTGAATATGTGTTGAAGTAGTCAGTAACTTATAGAGATAAGGTGTGTCT20.1589825119236884No Hit
ATTATGTTTCCGCTTTACTGAATAATTTTCATTATATTAAAAATGCTCGA20.1589825119236884No Hit
GCTTCGTAGTATGGGGTCATCACATATTTACGGTTGGCATAAATATTGAT20.1589825119236884No Hit
TGAAGACCCTCACAGGCAAAACCATCACCCTCGAGGTTGAAGCCTCTGAC20.1589825119236884No Hit
GAAATCATTTGTTAATTGTTTACTGTGCTGCCTGAAAGTTTGAGATTAGT20.1589825119236884No Hit
TGGAAAGGCAGTAAATTTCATTTTTTTCTCTGACACAATGTTTTGTAAAA20.1589825119236884No Hit
TTTCATAATTATTTTACTATTAAAGATTTGATTGGTGTGTTTATTGTTTG20.1589825119236884No Hit
CGTCACCGGCGAAGCCGGCTTTGCACATTCCTGAACCATTGTCCACAACA20.1589825119236884No Hit
GTCAGAAAACAAGACTCTGAAGTCCCAGTTTGGCAACGTCAAGCTCGATC20.1589825119236884No Hit
GGTGACTCGAAGAGCGATCCCCCTAAGGGTGCCAAGACCTTCTATGCCCA20.1589825119236884No Hit
CCCAGAACTGCTAGTACTACAGAAACTCATGGACTCTTTTCTTGTATTCA20.1589825119236884No Hit
TACTCTCGGTTTCCTGTATAACATTACTTTTAGCTGGGTTGAGTGCCAGG20.1589825119236884No Hit
GTCTTTAAACAAACTGCTATATGTGATAGTTGAAATAGTGTTACCTTCAT20.1589825119236884No Hit
GAGAAATCATGTTGGTGTAGTGTATGGGCAGTGTTCTGAGATTTGCGGGG20.1589825119236884No Hit
GAGATACCGATGCTTTAAAGGCAAAAAAGGACAATACTATAAACAAAGTT20.1589825119236884No Hit
GGTAAATTATAAACAATAACCATATACAGTAAGCTGTGTAAGTATACATA20.1589825119236884No Hit
CATGGATCTGACAGGTAAAAATGAAGGTATTGTACGTTAGCCATTCCTAT20.1589825119236884No Hit
GTTATTTCAATGTGTTTTATTTTATTTGATAATTGTTCATATATAGGTAG20.1589825119236884No Hit
GAAAATATGAGCTAATGAAGCCCTGTGAATGATTTGAAAAAAAGACAGAA20.1589825119236884No Hit
CTGAACGTGTTGTCAATAAGTTACAAAAGGAGGTTGACAGATTGGAAGAT20.1589825119236884No Hit
ACCATATCCAAACTGAAACCCTAATATATGTTTGTTTCTATTGATATATC20.1589825119236884No Hit
CTTCCCCACGCCATCTTGAGATTAGATTTGGCCGGACGTGATCTTACCGA20.1589825119236884No Hit
TGCAATGTAAGAATTTTTATTTGTGTTCCTGTGATGAATGAAAGTTAAGA20.1589825119236884No Hit
TTTTTAAATTTAATGACAAGAATATGATAATCAATATACATAACAACAAA20.1589825119236884No Hit
CCCAGTTCCAGATGTATCTGTTGTAGATCTTACTGTACGACTCGAGAAAC20.1589825119236884No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)