FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-6_S43_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-6_S43_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58497050
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG25881134.424347894466473Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3107780.531271235045186Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN668550.11428781451372333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3044500.0122.12771
GAGCGTC3212600.0115.297089
AGAGCGT3259350.0113.737148
AAGAGCG3343500.0110.984617
TCGGAAG3370000.0110.5309143
CGGAAGA3405100.0109.328424
ATCGGAA3413100.0109.314982
GAAGAGC3541450.0105.005726
GGAAGAG3774850.098.697835
CGCCGTA3155850.026.55378345-49
GGTCGCC3180350.026.37539740-44
GCCGTAT3211300.026.14372645-49
CCGTATC3215150.026.11630645-49
CTCGGTG3228850.026.00532235-39
TGGTCGC3231950.025.98738540-44
GTCGCCG3212300.025.98683240-44
GCGTCGT3198150.025.95029810-14
TCTCGGT3234400.025.93064735-39
CGTCGTG3197150.025.92975610-14
GTAGGGA3206250.025.86957415-19
Produced by FastQC (version 0.11.5)