FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-6_S43_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-6_S43_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58497050
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC24433464.176870457570082TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2871450.4908709071654041TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766370.1310100252918737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN622810.10646861679349644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2917100.0122.798781
TCGGAAG3190300.0112.507593
CGGAAGA3266950.0110.207954
ATCGGAA3272350.0109.6875462
GAAGAGC3294900.0109.016876
AGAGCAC3434050.0108.238538
GGAAGAG3398700.0105.820225
GAGCACA3563950.0104.364399
AAGAGCA3496200.0102.740027
GATCGGC228600.037.4673041
CGGCAGA253650.035.604054
TCGGCAG265000.033.292743
ATCGGCA296300.029.7881852
CGGGAGA149000.029.326174
GCAGAGC320550.028.4536976
GGCAGAG321000.028.3272485
TCGGGAG149800.027.5001183
TATGCCG3176550.026.35764745-49
CGTATGC3193800.026.1260240-44
GATCGGG145500.026.0987681
Produced by FastQC (version 0.11.5)