FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-5_S35_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-5_S35_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961561
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG915769.52368076492287Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT113231.1775643978905135Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26800.27871346695633453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.11782923808265934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA102200.0130.373891
GAGCGTC110700.0120.4665459
AGAGCGT111650.0119.4415368
AAGAGCG112350.0118.697367
TCGGAAG113500.0118.339923
ATCGGAA114050.0117.606092
CGGAAGA114150.0117.363744
GAAGAGC115450.0115.862846
GGAAGAG118900.0112.727295
CGGGTGG900.006383985753.637684145
CGGTTAG1052.4261499E-432.9774551
AGAGCGG2200.00581430329.25692145
GCGGCCT1205.3067977E-428.8552761
CCGTATC110900.026.98434345-49
GCCGTAT110900.026.98434345-49
CGCCGTA110800.026.97127245-49
GGTCGCC111000.026.94905540-44
CTCGGTG111050.026.94176935-39
TGGTCGC111450.026.85264240-44
GTAGGGA110050.026.84203115-19
Produced by FastQC (version 0.11.5)