FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-5_S35_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-5_S35_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961561
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC873239.081379132473135TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC107981.122965677684515TruSeq Adapter, Index 12 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26380.27434556933985466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.16026024349989237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA99650.0130.299741
TCGGAAG112050.0119.052323
ATCGGAA111500.0117.507182
CGGAAGA114300.0117.252724
GAAGAGC114800.0116.2424556
AGAGCAC115850.0116.142838
GGAAGAG116250.0114.911375
GAGCACA117550.0114.345679
AAGAGCA117500.0113.7476967
CTCGGAA3700.061.6654972
GCTCGGA4100.055.9766161
CGGGAGA2801.4551915E-1129.6067454
ATCGGGA2352.0972948E-929.4213222
GCCGTCT111300.026.7366645-49
TATGCCG111750.026.72798545-49
TGCCGTC112200.026.71938345-49
CGTATGC111950.026.70864940-44
TCGTATG112600.026.61585240-44
ATGCCGT112500.026.59896745-49
TCGGGAG2356.8641384E-826.457093
Produced by FastQC (version 0.11.5)