FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-4_S27_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2807.758381823219729Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT421.1637572734829593Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.24937655860349126No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1662510390689942No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.1662510390689942No Hit
TTTAGAATATTTAGAATAAAACATCATCCACAACCAATCATGTCAAGCCG60.1662510390689942No Hit
GACCTATATAAGGAATTAAGTGTGGTTCCATATTCCACATGTCGTGTCAA50.13854253255749516No Hit
CTTGTACGTGTAAAATAATTTTGACCAAGAAAATACTGTCAGTAGTTATG40.11083402604599613No Hit
TGAAAGTAACGCCAATGATAATTGAATTTACATTGTGGTCTCCGTATATC40.11083402604599613No Hit
CGGCAAGAAGGTCTGTTCGGACGTGGCACATTATCTTAAAGACAGGGCGA40.11083402604599613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACAT100.0076882956139.180567
GATCGGA351.0913936E-11139.180561
GAGCGTC553.8744474E-1088.569449
GAAGAGC553.8744474E-1088.569446
TCGGAAG553.8744474E-1088.569443
AGAGCGT553.8744474E-1088.569448
ATCGGAA553.8744474E-1088.569442
GGAAGAG553.8744474E-1088.569445
AAGAGCG607.748895E-1081.188667
CGGAAGA607.748895E-1081.188664
AGCGTCG551.585801E-450.611119
GTAGATC552.8921932E-1027.83611130-34
TGGTCGC552.8921932E-1027.83611140-44
GAGTGTA552.8921932E-1027.83611125-29
GTCGTGT552.8921932E-1027.83611110-14
GTCGCCG552.8921932E-1027.83611140-44
GTGTAGG552.8921932E-1027.83611115-19
TCGGTGG552.8921932E-1027.83611135-39
CCGTATC552.8921932E-1027.83611145-49
AGAGTGT552.8921932E-1027.83611125-29
Produced by FastQC (version 0.11.5)