FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-4_S27_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2526.982543640897755TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC421.1637572734829593TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.24937655860349126No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.24937655860349126No Hit
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC90.24937655860349126TruSeq Adapter, Index 12 (98% over 50bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.22166805209199225No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70.19395954558049322No Hit
GGGGCACATGTCATCATCTCATCCAGGTCCTGGTCTGTGGGGCTCTTACC60.1662510390689942No Hit
ATCAAAATACAAAATAAATTTGGGCTGCCAGGGTTACAGGAATAGTCTTA50.13854253255749516No Hit
TTCTCTGTTTGTTGTTTAAGAAATCTCCAACCGTCTCCTAAAATTCCAGT40.11083402604599613No Hit
ATCTGACCGACAGACACAGACGGCAGACCGACGATCCGACTAACTGACCG40.11083402604599613No Hit
TAAGGTTTCCCTCCATCAATAAAACTGGATATTCCCTATATAACCTGATA40.11083402604599613No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC40.11083402604599613TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCAT100.007704374139.083339
GATCGGA351.0913936E-11139.083331
AAGAGCA553.9108272E-1088.5075767
GAAGAGC553.9108272E-1088.5075766
TCGGAAG553.9108272E-1088.5075763
CGGAAGA553.9108272E-1088.5075764
AGAGCAC553.9108272E-1088.5075768
ATCGGAA553.9108272E-1088.5075762
GGAAGAG553.9108272E-1088.5075765
GAGCACA607.7852746E-1081.131949
AGCACAC551.594309E-450.5757569
ACACGTC503.3123797E-927.81666810-14
CACGTCT503.3123797E-927.81666810-14
TATGCCG552.928573E-1027.81666845-49
CCTTGTA552.928573E-1027.81666830-34
ACTCCAG552.928573E-1027.81666820-24
GTCTGAA552.928573E-1027.81666815-19
AACTCCA552.928573E-1027.81666820-24
TCTGCTT552.928573E-1027.81666855-59
CACCTTG552.928573E-1027.81666830-34
Produced by FastQC (version 0.11.5)