FastQCFastQC Report
Fri 15 Sep 2017
Geoduck-ctenidia-RNA-2_S11_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-2_S11_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031
Sequences flagged as poor quality0
Sequence length62-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG767.371483996120272Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.454898157129001No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT90.8729388942774006Illumina Single End PCR Primer 1 (100% over 50bp)
TAGTTATAAAAACTTCTTTCACATCAAAGTAAAATAAATAAATTAACGAA50.4849660523763337No Hit
GAATAGCTGGGTCTTTAATCAGTTGAATACATGGTGGTAGGTATTCAATG40.38797284190106696No Hit
TAGACGCTTACAGTAGGAGAATATCATTTTTAGTCAAGATCATGCCATTG40.38797284190106696No Hit
CTGGGCTCTAGCCACCAACAAACTATGTGATATCAACGTTAAAACACAAA30.2909796314258002No Hit
CGCGTACAGTGCTGAAATTACTGTTCTGTTACTCTTGTGTTATAATTGAA30.2909796314258002No Hit
GGCGGATCTACTTTTTAAAAAACCGGCGTAAGTTCCCTCTCCCATATACT30.2909796314258002No Hit
GGGAATTAGAAGTTTTTTTGAAGTTATTGAAAAATATGGTTTAAGAAGTT30.2909796314258002No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2909796314258002No Hit
GATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCCG30.2909796314258002Illumina Single End PCR Primer 1 (98% over 50bp)
GTTTTGTAGTATGATAGCAGACGTATCTGTTACATAAATTATACAACAAA30.2909796314258002No Hit
TAGTGCCCCTCCCACCAATACATGGGGAGGAAGTGCTGAAGTTGAAAACT30.2909796314258002No Hit
CCCTGATTATGTGTAGCTACATTTATATGATAACAATGCTCTGCCTTATT30.2909796314258002No Hit
TGTGTGTTTAAAATATCAGGTATTTAAATTTGACATAAGTTTATTCGAAA20.19398642095053348No Hit
CCCGAATCTAGCAATAAACATTTAATCGGCTGGATTACATGGGTACAATT20.19398642095053348No Hit
GGACACACTGGGAAGCCAGGTGAGAAAACAACAAAAATGACAGATGATAA20.19398642095053348No Hit
AAGTTTGACCATCACATCCAAAAAAAGTGTCAGATAGCTCATGTGCAGTT20.19398642095053348No Hit
TTTTATTATGAATTTATATAACTTGCTTTGACATACGTTGTGAATTTCCT20.19398642095053348No Hit
TCTGGATGTGTTTTCATGAGGCAGACAGTGGGTTTGATCTCAAGATGTGT20.19398642095053348No Hit
GTTTGATTAATTTTGAAATTTGAAATACAAGTATAAAAAAAATGATTATG20.19398642095053348No Hit
TTAAGCTTCATCGAGTGAAGACTGTAAGAAGAACGTTAGAAGAAGTTGGC20.19398642095053348No Hit
CCCGATAGTCCACGCGTATGGATCTGCCGTAGGGGGTCGTGAGATCAACA20.19398642095053348No Hit
GTTGGGGTGCTCATGGCGGTGGTGCTTTCTCCGGTAAAGATTTCTCCAAA20.19398642095053348No Hit
GTCACATTGGATTTTCTCCAACAGTTGATTGAAGGTACAAACGTGTGTAC20.19398642095053348No Hit
TTCAGAATGGATTTATATACATAGTGAAAATATTTAAATTACTACTTACA20.19398642095053348No Hit
GGACTCTATTTCTGACCATCTGGGTATTAGACAAATCTATTTCTGACCAT20.19398642095053348No Hit
GTTTGTTTGATATTGCGTGTCTGGTCTGTAACTTTGTCTTTAACTTGACT20.19398642095053348No Hit
TTTAAATGTCAATGTTTCTAATGACCAGTTTGGATATTATTATTACTATT20.19398642095053348No Hit
GGTAACTTGAACTCATTGCATATGAAATGACCACATAAAAATTGAAATCG20.19398642095053348No Hit
AGATCAAGTCCGCGGGAAATGGACAAACATGGCCACTATGACCAGCACAG20.19398642095053348No Hit
CTCGACCGGAGCGGTGACCGTCAAAACCGTCGCCGATCGAGCCACGGCAT20.19398642095053348No Hit
GCAGCGTGCATGACATAGATTCTCATCCTTGAGTGAGAAGAACAGCAAAA20.19398642095053348No Hit
GCAAAACGAGTTTAATTGTCAGCTATACAATAAACGACTATCCAACAGAA20.19398642095053348No Hit
GCTGGACAAAACACTTTCAACTCGTATCTCAAAAGTTTTGGCATGGCTTT20.19398642095053348No Hit
GAGTATTGCTATTTAGTATTAATGATAATCTGCAGATGTGGAGTTCTCGG20.19398642095053348No Hit
TTTCCCATTGGAACAGTAGCATACCTATATCTCTTGTGTATTGATATAAA20.19398642095053348No Hit
CCGTCCCCATTTAACCATCACATGTATATGGTAGTTAGTTTTAAGTATAG20.19398642095053348No Hit
GTCGATCTCTGCAGAGGGGTAGGAATCCTCCAAATGGGTGTCATGTTCAT20.19398642095053348No Hit
GTTACTTGAGTGATTTACTTTCAGATTTGTATTTTTGGTTGCAGGCCAAA20.19398642095053348No Hit
AGACGCTTTTTGCAACGAATCTATTCAACAACTTAAAATCTTTATTAAAA20.19398642095053348No Hit
GACCTACTGCATCGACAACGAGGCTCTCTACGATATCTGCTTCAGAACAC20.19398642095053348No Hit
ATCTTTAGGGACAGCAGAAGTTATATACGAGAGAAGATCAGATGCTGTTA20.19398642095053348No Hit
TAAGCTTTCAACTTTCTGTATGTATACTGAACGGAACAGCTAACCCACCA20.19398642095053348No Hit
CTTCCTTTCTAGATTTGACAGTAATTTTAGTAAAATTTTGTTTTTCATAA20.19398642095053348No Hit
GTGAAAACCTAATTTCTAATTTTTTTTTTTTTTTTGTTGTGTGTAATGGA20.19398642095053348No Hit
GTCGACGCAGCCGCGTACGTTCCCAGCAAACGCTACGTGTTTCTGTTCAG20.19398642095053348No Hit
ATTTCGAAACGTCAACTTTGTAAAGGACTGCAAAACCATGTAAACTGTGT20.19398642095053348No Hit
CAATGTTGTAGTTGTTATTTTTCAGTGTGCAATGATTTGTTAATTAACAA20.19398642095053348No Hit
CTTAGTCATTATTATCCTAAATGTGAGATGGCGGGTTATCTGTTCCGTTA20.19398642095053348No Hit
CGATGGTTTAACACCTAAAATGTATACACTATAGATATAAGAAGAAAAAT20.19398642095053348No Hit
GATCAATGCAGGCCCATGTAGAACAATTGTTATGTATTGAAATTTTTGCT20.19398642095053348No Hit
ACAGAAGCACGTCTGAAGGACCTGGCGCCACCTCTGCCAGTCATCTTCCT20.19398642095053348No Hit
TGAGACTGGAACCTTACAGTAATTTTATTTTGGCCTAAAATTTCAAGCAT20.19398642095053348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA100.005615523148.421051
GAGCGTC100.006602986141.09
AAGAGCG100.006602986141.07
TCGGAAG100.006602986141.03
CGGAAGA100.006602986141.04
AGAGCGT100.006602986141.08
ATCGGAA100.006602986141.02
GGAAGAG100.006602986141.05
AAAAAAA4004.0352208E-45.287500460-64
Produced by FastQC (version 0.11.5)