FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253470
Sequences flagged as poor quality0
Sequence length2-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAATCATAAACAAACGGGCAACATACCT3620.14281769045646428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTG202.6541427E-587.264632
CGCTTGT600.079.992583
CGGTTGT455.4569682E-1177.5685653
CCCGGTT455.4569682E-1177.5685651
ATAAATC70400.075.612822
TAAATCA75800.073.392093
CGTCCAC302.2600998E-672.720531
CCGCTTG553.6379788E-1271.398342
AATAAAT72900.070.0870361
CGATTGT258.022989E-569.811713
GTCCGTT501.382432E-1069.811711
CCGGTTG501.382432E-1069.811712
GTCCCCA501.382432E-1069.811711
TCATAAA87800.068.633577
AATCATA82850.068.332545
AAATCAT82550.067.351394
ATCATAA90050.067.086096
AACGTGC200.002901878265.448471
CGTGCAA200.002901878265.448473
GCTGCCC200.002901878265.448471
Produced by FastQC (version 0.11.2)