FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15909223
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4399000.075.91892
AATAAAT4467200.073.17141
TAAATCA4666700.072.8949053
AATCATA5075450.068.58365
TCATAAA5143700.068.237487
AAATCAT5062250.067.387434
ATCATAA5294150.066.791696
CATAAAC5589750.062.699518
ATAAACA6245050.056.137829
AGTAAAT31100.049.4138761
GTAAATC31800.048.901922
GATAAAT57850.046.4883351
GTCCCCA18300.043.9642141
GGCCCGT12050.043.1361271
AGAAATC45650.043.0767632
GTCCCCC10800.041.432561
AGAGCAC25550.040.69265794-95
CGCTTGT18550.040.21223
TGCCCGT194950.039.9709431
ATGAATC19350.039.9493522
Produced by FastQC (version 0.11.2)