FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15922255
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4460050.075.9450762
AATAAAT4523200.073.360461
TAAATCA4716900.072.74053
AATCATA5104350.068.7537165
TCATAAA5179950.068.526537
AAATCAT5098000.067.533484
ATCATAA5345950.066.7546546
CATAAAC5626750.062.9710168
ATAAACA6300550.056.299039
AGAAATC51550.051.327322
AGTAAAT29000.049.3480381
GTAAATC30150.049.270642
GATAAAT62150.048.2320371
GTGCAAC20250.044.594842
GTCCCCA17400.044.1070821
CGCTTGT19500.043.7666933
AGTCATA14400.043.281145
CGTGCAA20900.043.200861
CCGCTTG19150.041.7334332
AAGAAAT61650.041.593351
Produced by FastQC (version 0.11.2)