FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15952602
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4382600.075.6766052
AATAAAT4491950.073.11791
TAAATCA4640600.072.3599853
AATCATA4967350.067.99865
TCATAAA5020450.067.63037
AAATCAT4961950.066.853524
ATCATAA5182200.065.970856
GTAAATC46050.065.9292142
AGTAAAT46800.064.864311
CATAAAC5450000.062.2162558
ATAAACA6107000.055.348859
AAATCAG48700.046.575844
GTCCCCA20700.044.5186731
AGAAATC46350.044.058222
GTCCCCC10300.041.2261731
ATGAATC20500.041.2122232
TGCCCGT207850.040.0550881
AGAGCAC25950.038.8919694-95
TTGCCCG118550.037.609541
GATAAAT49650.037.3032761
Produced by FastQC (version 0.11.2)