FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15956775
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4407550.075.639132
AATAAAT4510650.073.182531
TAAATCA4650400.072.276773
AATCATA5015200.068.152615
TCATAAA5061950.067.652617
AAATCAT5024450.066.9375154
ATCATAA5223000.066.030066
CATAAAC5494250.062.2062038
ATAAACA6150350.055.5879679
ATGAATC26150.047.5563132
GTCCCCA17650.042.8829771
TGCCCGT213800.042.2500271
AGAAATC41600.042.025142
AGAGCAC26000.039.5314294-95
GTAAATC23350.039.1723752
GATAAAT46600.038.1884081
AGTAAAT24000.037.5438731
GTCCCCC9250.036.529181
TTGCCCG119900.036.222481
GAATCAT36500.036.1647574
Produced by FastQC (version 0.11.2)