FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_CTTGTA_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_CTTGTA_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10548081
Sequences flagged as poor quality0
Sequence length1-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT859800.069.269791
ATAAATC904500.067.125252
TAAATCA980700.062.874073
TCATAAA1112350.056.8471457
AATCATA1126300.055.856185
ATCATAA1219700.052.4081736
AAATCAT1185550.052.2591484
AGAGCAC126150.047.52691394-95
CATAAAC1396400.045.4667748
ATAAACA1493250.042.4836359
GAGCACA71600.041.10622494-95
AAGAGCA195900.033.62651494-95
GTCCCCA21150.031.1639231
ACAAACG1169700.028.00875512-13
TGATACG15650.027.5141893
GTCCCCC14850.026.872541
GAAGAGC266350.026.34010194-95
AAACGGG1232450.026.20290414-15
CCCCGCA37750.025.8931391
CAAACGG1211600.025.80275214-15
Produced by FastQC (version 0.11.2)