FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_CTTGTA_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_CTTGTA_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15881522
Sequences flagged as poor quality0
Sequence length1-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1318650.069.041011
ATAAATC1384650.066.988692
TAAATCA1499800.062.495683
TCATAAA1686450.057.364787
AATCATA1716900.055.715915
ATCATAA1854100.052.5727776
AAATCAT1790100.052.400764
AGAGCAC194050.047.73171294-95
CATAAAC2125000.045.7105038
ATAAACA2274650.042.647199
GAGCACA111400.040.27176394-95
AAGAGCA295200.033.63618594-95
TGATACG26200.029.0785753
ACAAACG1796850.028.0071412-13
GTCCCCA27800.027.7161241
AAACGGG1890900.026.2614514-15
CAAACGG1859000.025.72212614-15
GAAGAGC394050.025.57297594-95
ATACGGC30450.025.0271745
GATACGG30600.024.9008774
Produced by FastQC (version 0.11.2)