FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_CTTGTA_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_CTTGTA_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15906198
Sequences flagged as poor quality0
Sequence length1-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1280100.068.9110951
ATAAATC1345750.066.3612442
TAAATCA1477700.061.2263683
TCATAAA1639600.056.0374577
AATCATA1665450.054.880475
ATCATAA1796300.051.6184546
AAATCAT1747150.051.3122184
AGAGCAC188850.047.5920194-95
CATAAAC2070900.044.548698
ATAAACA2229350.041.367899
GAGCACA111350.039.56677694-95
AAGAGCA293400.033.1107894-95
GTCCCCA32200.030.966991
TGATACG24100.029.3188823
ACAAACG1730450.027.74350212-13
GAAGAGC394600.026.11942794-95
AAACGGG1818050.026.072614-15
CAAACGG1794200.025.45766614-15
ATACGGC28950.024.4168455
ACGGGCA1970550.024.12372416-17
Produced by FastQC (version 0.11.2)