FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15922161
Sequences flagged as poor quality0
Sequence length1-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1289250.068.693411
ATAAATC1359550.066.1130142
TAAATCA1470550.061.5160753
TCATAAA1649300.055.716517
AATCATA1693850.054.343725
AAATCAT1771500.051.109284
ATCATAA1824200.050.915446
AGAGCAC183050.048.49661394-95
CATAAAC2081850.044.3589748
ATAAACA2222650.041.560729
GAGCACA105100.039.8739694-95
AAGAGCA285500.033.78125894-95
GTCCCCA30850.028.0822721
ACAAACG1729750.027.68341312-13
GAAGAGC390050.026.75739194-95
AAACGGG1822700.025.92667414-15
CCCCCGA47650.025.4912821
CAAACGG1795600.025.31748414-15
TGATACG23150.025.0800573
ACGGGCA1978450.023.88626316-17
Produced by FastQC (version 0.11.2)