FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827107
Sequences flagged as poor quality0
Sequence length1-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC13400.054.162835
AGAGCAC80450.046.78617594-95
TGATACG16400.042.216443
TACGGCG17650.042.0732966
GATACGG18500.038.5630844
ATGATAC18250.038.1390532
AATGATA19450.035.748511
AAGAGCA108700.034.43562794-95
AATCATA23950.031.3313035
GAGCACA61950.030.77054494-95
TCTTACG21200.029.2035264
ACGGCGA23900.028.6911037
GAAGAGC138350.027.45650194-95
TCATAAA28800.027.0888027
GGCGACC22700.025.5371259
AGCCGCC10550.025.199361
CCCATTT29850.023.6274595
ATCATAA35350.022.3996126
CCGGAAG8100.022.2539143
GGAAGAG179900.021.98205494-95
Produced by FastQC (version 0.11.2)