FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_GCCAAT_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_GCCAAT_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316692
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1218000.064.105845
TAAATCA1113400.062.6740043
ATAAATC1105200.062.4621352
ATCATAA1286050.061.858846
TCATAAA1277800.061.8199927
AAATCAT1230900.061.267184
CATAAAC1294800.060.7267078
AATAAAT1361500.050.7190631
ATAAACA1581550.049.7442869
AACCGGG7150.044.671731
ACACGGG9800.039.496752
CACACGG8600.038.709161
CACGGGC9900.038.2013783
CCAACGG10650.037.6122552
ACCGGGC8400.037.50662
GACCGTT11350.034.8960082
AGAGCAC55150.034.4432394-95
AAACGGG1161050.034.2589214-15
AGAAATC27350.033.8999372
ACAAACG1195850.033.74135212-13
Produced by FastQC (version 0.11.2)