FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13494573
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA2183400.063.26075
ATAAATC2061500.062.227262
TAAATCA2068200.061.8948363
TCATAAA2328850.061.4875537
ATCATAA2351150.061.351576
AAATCAT2252750.060.514184
CATAAAC2363000.060.4431848
AATAAAT2496950.051.222191
ATAAACA2886050.049.5937849
CACACGG16500.040.6929781
ACACGGG18900.036.013612
AGAGCAC101000.035.6711394-95
AACCGGG12300.035.3003271
AAACGGG2133800.034.24767714-15
ACAAACG2192400.033.8048112-13
ACGGGCA2186550.033.24141316-17
CAAACGG2129200.033.2341314-15
CCAACGG16650.033.080782
AGAAATC52050.032.6064382
GGGCAAC2212750.032.45468518-19
Produced by FastQC (version 0.11.2)