FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_CAGATC_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_CAGATC_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6056165
Sequences flagged as poor quality0
Sequence length1-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC78360.12938881288736354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA630600.057.9352345
TCATAAA669900.055.5572787
ATCATAA688600.054.5218356
TAAATCA616000.053.444313
AAATCAT663400.053.3599854
CATAAAC713750.052.0275968
ATAAATC671700.048.001372
CACGGGC7750.047.469713
CACACGG7850.044.5482641
CCGGGCA7050.043.9101033
AGAGCAC66150.043.87751494-95
AACCGGG6250.043.7578161
ACCGGGC6700.043.5109982
AATAAAT744200.043.309651
ATAAACA893350.041.6753169
CCAACGG8300.036.2040562
TACGGCG7700.034.385946
GAGCACA40800.034.19165894-95
ACACGGG10550.034.0093272
ATACGGC7850.033.7246935
Produced by FastQC (version 0.11.2)