FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_CAGATC_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_CAGATC_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468557
Sequences flagged as poor quality0
Sequence length1-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC158380.12702352004325762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1253800.056.9320955
TCATAAA1346550.055.185457
ATCATAA1388550.053.967136
AAATCAT1331550.052.7926984
TAAATCA1228150.052.6108863
CATAAAC1444000.051.307388
ATAAATC1350700.047.8329282
AGAGCAC133000.043.40451494-95
AATAAAT1486300.043.1663781
ATAAACA1801300.041.300359
ACCGGGC13700.041.206962
AACCGGG15000.038.810471
CCGGGCA15350.037.9539533
CACACGG17750.036.834211
CACGGGC17800.036.5064853
AGCCCCC12250.035.0939251
GAGCACA82100.033.3239894-95
CCAACGG14300.033.2116472
AGCCCGC6000.032.839631
AAACGGG1181300.032.39462714-15
Produced by FastQC (version 0.11.2)