FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12742235
Sequences flagged as poor quality0
Sequence length1-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC174650.13706386673923374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1269200.056.68185
TCATAAA1352450.054.425027
ATCATAA1389650.053.4047286
TAAATCA1271850.052.750883
AAATCAT1350650.052.176724
CATAAAC1450400.050.736158
ATAAATC1396550.048.070762
AGAGCAC132100.043.98478394-95
AATAAAT1536700.043.707231
ATAAACA1817450.040.633389
GAGCACA83150.034.70934394-95
AACCGGG9550.033.205291
ACCGGGC10000.032.614972
AGCCCGA10150.032.1224821
TACGGCG12500.031.821916
AGCCGCC12000.031.6366141
AAACGGG1182500.031.54852714-15
AGCCCCC12400.031.3364541
CACGGGC13400.031.0111873
CAAACGG1174550.030.74073814-15
Produced by FastQC (version 0.11.2)