FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_ATCACG_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_ATCACG_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173081
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA141000.061.42355
ATCATAA147900.059.1268276
CATAAAC146350.059.0990378
TCATAAA147300.058.7331287
TAAATCA131850.056.32663
AAATCAT149700.056.182934
ATAAATC132600.051.279792
ATAAACA188950.045.9995169
TACGGCG1151.8189894E-1242.7421886
AATAAAT168050.040.335371
AGAGCAC6750.040.14421594-95
AGGCCCC902.0765583E-839.6656231
ACCGGGC1051.884473E-938.2686422
GCACGTT1600.036.2568551
AAACGGG120800.036.0817714-15
ACGGGCA121900.035.5349816-17
GGGCAAC122550.035.1345718-19
ATACGGC1402.3646862E-1135.0946125
CAAACGG122250.034.93963214-15
CCAACGG1304.0017767E-1034.343652
Produced by FastQC (version 0.11.2)