FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_ATCACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_ATCACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13657433
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1552000.059.2688145
ATCATAA1670950.057.9046
CATAAAC1677150.057.1962748
TCATAAA1689600.056.810417
TAAATCA1513100.056.378973
AAATCAT1642950.054.8311654
ATAAATC1582200.054.0249942
ATAAACA2135400.045.0918439
AATAAAT2006200.042.5741041
AGAGCAC88700.038.8163794-95
AAACGGG1363900.035.42047514-15
ACGGGCA1370100.035.05807516-17
GGGCAAC1373800.034.53832218-19
CAAACGG1369400.034.4099514-15
AACGGGC1365400.033.68534516-17
CGGGCAA1367350.033.3577518-19
GCAACAT1437050.033.05937620-21
ACAAACG1516550.032.43145412-13
GGCAACA1417900.031.94311120-21
CATACCT1549800.030.19459524-25
Produced by FastQC (version 0.11.2)