FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_ACAGTG_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_ACAGTG_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889732
Sequences flagged as poor quality0
Sequence length2-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC28570.3211079291292209No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA15380.17286104130232474No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT9720.10924637980875139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA47050.064.2636645
TCATAAA52050.058.2186627
ATCATAA54150.056.343726
CACACGG1500.056.3350751
AAATCAT56200.052.6063424
AACCGGG853.6379788E-1252.3236051
CACGGGC1800.051.9314773
TAAATCA51550.051.7227973
ACCGGGC1000.048.9418752
CATAAAC62600.048.275588
AATAAAT55750.045.232931
CCAACGG2050.043.407432
ATAAACA70700.043.263749
CCGGGCA1151.8189894E-1242.5773543
GCCGGGA450.001327600139.5489922
AGAGCAC8900.039.5292994-95
ATACGGC2200.038.4579625
ACACGGG2500.037.3737952
ATAAATC67800.037.077182
TACGGCG2300.036.794186
Produced by FastQC (version 0.11.2)