FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11370712
Sequences flagged as poor quality0
Sequence length1-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC511740.4500509730613176No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA269350.23688050493232085No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT156240.13740564355160872No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG147160.1294202157261568No Hit
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA119960.10549911034594844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA567850.058.893125
TCATAAA629300.054.5785037
ATCATAA650700.053.322666
TAAATCA602100.051.106573
AAATCAT668200.049.3457074
CATAAAC767700.044.9860538
AATAAAT700500.043.9067881
AGAGCAC108950.040.79603694-95
ATAAACA885800.039.2315679
ATAAATC882100.034.982112
AACCGGG12250.030.4333531
AAGAGCA165950.030.05801894-95
AGCCCCC16250.030.043181
ACCGGGC13250.029.4880312
AGCCGCC20050.029.440411
GAGCACA73000.028.78279794-95
CACGGGC17850.028.6110923
AAACGGG634600.027.59478214-15
GCCCGTA17950.027.4560182
AGCCCGT10100.027.2443771
Produced by FastQC (version 0.11.2)