Processing single-end Bismark output file(s) (SAM format): 1_ATCACG_L001_R1_001_bismark_bt2.bam 2_CGATGT_L001_R1_001_bismark_bt2.bam 3_TTAGGC_L001_R1_001_bismark_bt2.bam 4_TGACCA_L001_R1_001_bismark_bt2.bam 5_ACAGTG_L001_R1_001_bismark_bt2.bam 6_GCCAAT_L001_R1_001_bismark_bt2.bam 7_CAGATC_L001_R1_001_bismark_bt2.bam 8_ACTTGA_L001_R1_001_bismark_bt2.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>1_ATCACG_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 1_ATCACG_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 1_ATCACG_L001_R1_001_bismark_bt2.bam: 618 Total number duplicated alignments removed: 5 (0.81%) Duplicated alignments were found at: 5 different position(s) Total count of deduplicated leftover sequences: 613 (99.19% of total) Checking file >>2_CGATGT_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 2_CGATGT_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 2_CGATGT_L001_R1_001_bismark_bt2.bam: 594 Total number duplicated alignments removed: 4 (0.67%) Duplicated alignments were found at: 4 different position(s) Total count of deduplicated leftover sequences: 590 (99.33% of total) Checking file >>3_TTAGGC_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 3_TTAGGC_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 3_TTAGGC_L001_R1_001_bismark_bt2.bam: 623 Total number duplicated alignments removed: 4 (0.64%) Duplicated alignments were found at: 4 different position(s) Total count of deduplicated leftover sequences: 619 (99.36% of total) Checking file >>4_TGACCA_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 4_TGACCA_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 4_TGACCA_L001_R1_001_bismark_bt2.bam: 615 Total number duplicated alignments removed: 4 (0.65%) Duplicated alignments were found at: 4 different position(s) Total count of deduplicated leftover sequences: 611 (99.35% of total) Checking file >>5_ACAGTG_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 5_ACAGTG_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 5_ACAGTG_L001_R1_001_bismark_bt2.bam: 623 Total number duplicated alignments removed: 4 (0.64%) Duplicated alignments were found at: 4 different position(s) Total count of deduplicated leftover sequences: 619 (99.36% of total) Checking file >>6_GCCAAT_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 6_GCCAAT_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 6_GCCAAT_L001_R1_001_bismark_bt2.bam: 608 Total number duplicated alignments removed: 5 (0.82%) Duplicated alignments were found at: 5 different position(s) Total count of deduplicated leftover sequences: 603 (99.18% of total) Checking file >>7_CAGATC_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 7_CAGATC_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 7_CAGATC_L001_R1_001_bismark_bt2.bam: 602 Total number duplicated alignments removed: 3 (0.50%) Duplicated alignments were found at: 3 different position(s) Total count of deduplicated leftover sequences: 599 (99.50% of total) Checking file >>8_ACTTGA_L001_R1_001_bismark_bt2.bam<< for signs of file truncation... Output file is: 8_ACTTGA_L001_R1_001_bismark_bt2.deduplicated.bam Total number of alignments analysed in 8_ACTTGA_L001_R1_001_bismark_bt2.bam: 621 Total number duplicated alignments removed: 2 (0.32%) Duplicated alignments were found at: 2 different position(s) Total count of deduplicated leftover sequences: 619 (99.68% of total)