Bismark report for: data/7_CAGATC_L001_R1_001.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Orange_block/wd/18-06-13/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000 Number of alignments with a unique best hit from the different alignments: 602 Mapping efficiency: 60.2% Sequences with no alignments under any condition: 99 Sequences did not map uniquely: 299 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 279 ((converted) top strand) CT/GA: 279 ((converted) bottom strand) GA/CT: 20 (complementary to (converted) top strand) GA/GA: 24 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5354 Total methylated C's in CpG context: 271 Total methylated C's in CHG context: 42 Total methylated C's in CHH context: 254 Total methylated C's in Unknown context: 11 Total unmethylated C's in CpG context: 620 Total unmethylated C's in CHG context: 989 Total unmethylated C's in CHH context: 3178 Total unmethylated C's in Unknown context: 31 C methylated in CpG context: 30.4% C methylated in CHG context: 4.1% C methylated in CHH context: 7.4% C methylated in Unknown context (CN or CHN): 26.2% Bismark completed in 0d 0h 1m 38s