Bismark report for: data/4_TGACCA_L001_R1_001.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Orange_block/wd/18-06-13/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000 Number of alignments with a unique best hit from the different alignments: 615 Mapping efficiency: 61.5% Sequences with no alignments under any condition: 91 Sequences did not map uniquely: 294 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 291 ((converted) top strand) CT/GA: 295 ((converted) bottom strand) GA/CT: 14 (complementary to (converted) top strand) GA/GA: 15 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5571 Total methylated C's in CpG context: 279 Total methylated C's in CHG context: 41 Total methylated C's in CHH context: 270 Total methylated C's in Unknown context: 8 Total unmethylated C's in CpG context: 616 Total unmethylated C's in CHG context: 1054 Total unmethylated C's in CHH context: 3311 Total unmethylated C's in Unknown context: 42 C methylated in CpG context: 31.2% C methylated in CHG context: 3.7% C methylated in CHH context: 7.5% C methylated in Unknown context (CN or CHN): 16.0% Bismark completed in 0d 0h 1m 41s