Bismark report for: data/2_CGATGT_L001_R1_001.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Orange_block/wd/18-06-13/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000 Number of alignments with a unique best hit from the different alignments: 594 Mapping efficiency: 59.4% Sequences with no alignments under any condition: 105 Sequences did not map uniquely: 301 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 296 ((converted) top strand) CT/GA: 263 ((converted) bottom strand) GA/CT: 18 (complementary to (converted) top strand) GA/GA: 17 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5252 Total methylated C's in CpG context: 234 Total methylated C's in CHG context: 52 Total methylated C's in CHH context: 316 Total methylated C's in Unknown context: 12 Total unmethylated C's in CpG context: 568 Total unmethylated C's in CHG context: 971 Total unmethylated C's in CHH context: 3111 Total unmethylated C's in Unknown context: 35 C methylated in CpG context: 29.2% C methylated in CHG context: 5.1% C methylated in CHH context: 9.2% C methylated in Unknown context (CN or CHN): 25.5% Bismark completed in 0d 0h 1m 41s