Bismark report for: data/1_ATCACG_L001_R1_001.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Orange_block/wd/18-06-13/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000 Number of alignments with a unique best hit from the different alignments: 618 Mapping efficiency: 61.8% Sequences with no alignments under any condition: 95 Sequences did not map uniquely: 287 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 299 ((converted) top strand) CT/GA: 279 ((converted) bottom strand) GA/CT: 21 (complementary to (converted) top strand) GA/GA: 19 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5652 Total methylated C's in CpG context: 266 Total methylated C's in CHG context: 44 Total methylated C's in CHH context: 239 Total methylated C's in Unknown context: 21 Total unmethylated C's in CpG context: 589 Total unmethylated C's in CHG context: 1047 Total unmethylated C's in CHH context: 3467 Total unmethylated C's in Unknown context: 29 C methylated in CpG context: 31.1% C methylated in CHG context: 4.0% C methylated in CHH context: 6.4% C methylated in Unknown context (CN or CHN): 42.0% Bismark completed in 0d 0h 1m 40s