Alignment Stats
| Sequences analysed in total |
47401736 |
| Single-end alignments with a unique best hit |
15838795 |
| Sequences without alignments under any condition |
13874948 |
| Sequences that did not map uniquely |
17687993 |
| Genomic sequence context not extractable (edges of chromosomes) |
13185 |
|
|
Cytosine Methylation
| Total C's analysed |
93616428 |
| Methylated C's in CpG context |
9995135 |
| Methylated C's in CHG context |
402878 |
| Methylated C's in CHH context |
5641348 |
| Methylated C's in Unknown context |
2165 |
| Unmethylated C's in CpG context |
3094855 |
| Unmethylated C's in CHG context |
18627231 |
| Unmethylated C's in CHH context |
55854981 |
| Unmethylated C's in Unknown context |
15434 |
| Percentage methylation (CpG context) |
76.4% |
| Percentage methylation (CHG context) |
2.1% |
| Percentage methylation (CHH context) |
9.2% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
3592897 |
original top strand |
| CTOT |
4352516 |
complementary to original top strand |
| CTOB |
4308552 |
complementary to original bottom strand |
| OB |
3571645 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
15825610 |
| Unique alignments |
11859086 |
| Duplicates removed |
3966524 |
| Duplicated alignments were found at 1843603 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
69420697 |
| Methylated C's in CpG context |
6725035 |
| Methylated C's in CHG context |
308623 |
| Methylated C's in CHH context |
4618857 |
| Unmethylated C's in CpG context |
2383446 |
| Unmethylated C's in CHG context |
13280520 |
| Unmethylated C's in CHH context |
42104216 |
| Percentage methylation (CpG context) |
73.8% |
| Percentage methylation (CHG context) |
2.3% |
| Percentage methylation (CHH context) |
9.9% |
|
|
M-Bias Plot