Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 18170956
Sequences without alignments under any condition 15404219
Sequences that did not map uniquely 21472931
Genomic sequence context not extractable (edges of chromosomes) 16272

Cytosine Methylation

Total C's analysed 109733028
Methylated C's in CpG context 14029679
Methylated C's in CHG context 448447
Methylated C's in CHH context 5906339
Methylated C's in Unknown context 2550
Unmethylated C's in CpG context 2832392
Unmethylated C's in CHG context 22328360
Unmethylated C's in CHH context 64187811
Unmethylated C's in Unknown context 18670
Percentage methylation (CpG context) 83.2%
Percentage methylation (CHG context) 2.0%
Percentage methylation (CHH context) 8.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3982167 original top strand
CTOT 5128139 complementary to original top strand
CTOB 5088474 complementary to original bottom strand
OB 3955904 original bottom strand

Deduplication

Alignments analysed 18154684
Unique alignments 12104246
Duplicates removed 6050438
Duplicated alignments were found at 2434465 different positions

Cytosine Methylation after Extraction

Total C's analysed 72146273
Methylated C's in CpG context 8198593
Methylated C's in CHG context 315055
Methylated C's in CHH context 4549990
Unmethylated C's in CpG context 1888052
Unmethylated C's in CHG context 13985858
Unmethylated C's in CHH context 43208725
Percentage methylation (CpG context) 81.3%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.5%

M-Bias Plot