Alignment Stats
| Sequences analysed in total |
55048106 |
| Single-end alignments with a unique best hit |
18170956 |
| Sequences without alignments under any condition |
15404219 |
| Sequences that did not map uniquely |
21472931 |
| Genomic sequence context not extractable (edges of chromosomes) |
16272 |
|
|
Cytosine Methylation
| Total C's analysed |
109733028 |
| Methylated C's in CpG context |
14029679 |
| Methylated C's in CHG context |
448447 |
| Methylated C's in CHH context |
5906339 |
| Methylated C's in Unknown context |
2550 |
| Unmethylated C's in CpG context |
2832392 |
| Unmethylated C's in CHG context |
22328360 |
| Unmethylated C's in CHH context |
64187811 |
| Unmethylated C's in Unknown context |
18670 |
| Percentage methylation (CpG context) |
83.2% |
| Percentage methylation (CHG context) |
2.0% |
| Percentage methylation (CHH context) |
8.4% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
3982167 |
original top strand |
| CTOT |
5128139 |
complementary to original top strand |
| CTOB |
5088474 |
complementary to original bottom strand |
| OB |
3955904 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
18154684 |
| Unique alignments |
12104246 |
| Duplicates removed |
6050438 |
| Duplicated alignments were found at 2434465 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
72146273 |
| Methylated C's in CpG context |
8198593 |
| Methylated C's in CHG context |
315055 |
| Methylated C's in CHH context |
4549990 |
| Unmethylated C's in CpG context |
1888052 |
| Unmethylated C's in CHG context |
13985858 |
| Unmethylated C's in CHH context |
43208725 |
| Percentage methylation (CpG context) |
81.3% |
| Percentage methylation (CHG context) |
2.2% |
| Percentage methylation (CHH context) |
9.5% |
|
|
M-Bias Plot