Alignment Stats
| Sequences analysed in total |
54458572 |
| Single-end alignments with a unique best hit |
15680143 |
| Sequences without alignments under any condition |
16326025 |
| Sequences that did not map uniquely |
22452404 |
| Genomic sequence context not extractable (edges of chromosomes) |
18576 |
|
|
Cytosine Methylation
| Total C's analysed |
100296882 |
| Methylated C's in CpG context |
17662617 |
| Methylated C's in CHG context |
491939 |
| Methylated C's in CHH context |
5196171 |
| Methylated C's in Unknown context |
3246 |
| Unmethylated C's in CpG context |
2900560 |
| Unmethylated C's in CHG context |
19823190 |
| Unmethylated C's in CHH context |
54222405 |
| Unmethylated C's in Unknown context |
19966 |
| Percentage methylation (CpG context) |
85.9% |
| Percentage methylation (CHG context) |
2.4% |
| Percentage methylation (CHH context) |
8.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
3528573 |
original top strand |
| CTOT |
4336953 |
complementary to original top strand |
| CTOB |
4298059 |
complementary to original bottom strand |
| OB |
3497982 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
15661567 |
| Unique alignments |
8177897 |
| Duplicates removed |
7483670 |
| Duplicated alignments were found at 1903267 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
50769500 |
| Methylated C's in CpG context |
7110525 |
| Methylated C's in CHG context |
280561 |
| Methylated C's in CHH context |
3592530 |
| Unmethylated C's in CpG context |
1619619 |
| Unmethylated C's in CHG context |
9193433 |
| Unmethylated C's in CHH context |
28972832 |
| Percentage methylation (CpG context) |
81.4% |
| Percentage methylation (CHG context) |
3.0% |
| Percentage methylation (CHH context) |
11.0% |
|
|
M-Bias Plot