Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L002_R1_001_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/reads/trimmed/sl2/Sealice_F2_S22_L002_R2_001_val_2.fq.gz

Data processed at 15:57 on 2020-02-04


Alignment Stats

Sequence pairs analysed in total 81606018
Paired-end alignments with a unique best hit 17584142
Pairs without alignments under any condition 51170344
Pairs that did not map uniquely 12851532
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 761182841
Methylated C's in CpG context 1619428
Methylated C's in CHG context 1312509
Methylated C's in CHH context 7923134
Methylated C's in Unknown context 866500
Unmethylated C's in CpG context 122527945
Unmethylated C's in CHG context 127216928
Unmethylated C's in CHH context 500582897
Unmethylated C's in Unknown context 6668909
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4441844 original top strand
CTOT 4370400 complementary to original top strand
CTOB 4356733 complementary to original bottom strand
OB 4415163 original bottom strand

Deduplication

Alignments analysed 17584140
Unique alignments 14322894
Duplicates removed 3261246
Duplicated alignments were found at 2198526 different positions

Cytosine Methylation after Extraction

Total C's analysed 365713938
Methylated C's in CpG context 721022
Methylated C's in CHG context 627972
Methylated C's in CHH context 3869671
Unmethylated C's in CpG context 58117318
Unmethylated C's in CHG context 60230769
Unmethylated C's in CHH context 242147186
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.6%

M-Bias Plot