WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4077713
total bases: 587190672
Q20 bases: 576138982(98.1179%)
Q30 bases: 558663553(95.1418%)

Read1 after filtering:
total reads: 4073573
total bases: 586592012
Q20 bases: 575710680(98.145%)
Q30 bases: 558300172(95.1769%)

Filtering result:
reads passed filter: 4073573
reads failed due to low quality: 830
reads failed due to too many N: 3310
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.8215%

JSON report: SewardAK_Q326.json
HTML report: SewardAK_Q326.html

fastp -i SewardAK_Q326.F.fq.gz -o SewardAK_Q326.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q326.json -h SewardAK_Q326.html 
fastp v0.20.0, time used: 41 seconds