WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4209483
total bases: 606165552
Q20 bases: 594610064(98.0937%)
Q30 bases: 576476982(95.1022%)

Read1 after filtering:
total reads: 4205167
total bases: 605541318
Q20 bases: 594162621(98.1209%)
Q30 bases: 576097140(95.1375%)

Filtering result:
reads passed filter: 4205167
reads failed due to low quality: 869
reads failed due to too many N: 3447
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 80.9201%

JSON report: SewardAK_Q324.json
HTML report: SewardAK_Q324.html

fastp -i SewardAK_Q324.F.fq.gz -o SewardAK_Q324.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q324.json -h SewardAK_Q324.html 
fastp v0.20.0, time used: 44 seconds