WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2491116
total bases: 358720704
Q20 bases: 352033189(98.1357%)
Q30 bases: 341412415(95.175%)

Read1 after filtering:
total reads: 2488446
total bases: 358334763
Q20 bases: 351758939(98.1649%)
Q30 bases: 341180861(95.2129%)

Filtering result:
reads passed filter: 2488446
reads failed due to low quality: 589
reads failed due to too many N: 2081
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.7434%

JSON report: SewardAK_Q323.json
HTML report: SewardAK_Q323.html

fastp -i SewardAK_Q323.F.fq.gz -o SewardAK_Q323.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q323.json -h SewardAK_Q323.html 
fastp v0.20.0, time used: 30 seconds