WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4147891
total bases: 597296304
Q20 bases: 586282933(98.1561%)
Q30 bases: 568835739(95.2351%)

Read1 after filtering:
total reads: 4143742
total bases: 596695704
Q20 bases: 585850391(98.1824%)
Q30 bases: 568465673(95.2689%)

Filtering result:
reads passed filter: 4143742
reads failed due to low quality: 801
reads failed due to too many N: 3348
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.666%

JSON report: SewardAK_Q318.json
HTML report: SewardAK_Q318.html

fastp -i SewardAK_Q318.F.fq.gz -o SewardAK_Q318.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q318.json -h SewardAK_Q318.html 
fastp v0.20.0, time used: 42 seconds