WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3179105
total bases: 457791120
Q20 bases: 449008182(98.0815%)
Q30 bases: 435389743(95.1066%)

Read1 after filtering:
total reads: 3175976
total bases: 457338471
Q20 bases: 448679302(98.1066%)
Q30 bases: 435107848(95.1391%)

Filtering result:
reads passed filter: 3175976
reads failed due to low quality: 582
reads failed due to too many N: 2547
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.1716%

JSON report: SewardAK_Q316.json
HTML report: SewardAK_Q316.html

fastp -i SewardAK_Q316.F.fq.gz -o SewardAK_Q316.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q316.json -h SewardAK_Q316.html 
fastp v0.20.0, time used: 36 seconds