WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3802801
total bases: 547603344
Q20 bases: 536780797(98.0237%)
Q30 bases: 520178376(94.9918%)

Read1 after filtering:
total reads: 3798882
total bases: 547035626
Q20 bases: 536372298(98.0507%)
Q30 bases: 519830543(95.0268%)

Filtering result:
reads passed filter: 3798882
reads failed due to low quality: 759
reads failed due to too many N: 3160
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 82.4648%

JSON report: SewardAK_Q314.json
HTML report: SewardAK_Q314.html

fastp -i SewardAK_Q314.F.fq.gz -o SewardAK_Q314.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q314.json -h SewardAK_Q314.html 
fastp v0.20.0, time used: 41 seconds