WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1915594
total bases: 275845536
Q20 bases: 260900977(94.5823%)
Q30 bases: 238505877(86.4636%)

Read1 after filtering:
total reads: 1914860
total bases: 274863101
Q20 bases: 260676707(94.8387%)
Q30 bases: 238466784(86.7584%)

Filtering result:
reads passed filter: 1914860
reads failed due to low quality: 482
reads failed due to too many N: 252
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 58.0102%

JSON report: SewardAK_Q303.json
HTML report: SewardAK_Q303.html

fastp -i SewardAK_Q303.F.fq.gz -o SewardAK_Q303.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q303.json -h SewardAK_Q303.html 
fastp v0.20.0, time used: 28 seconds