WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1956377
total bases: 281718288
Q20 bases: 274262257(97.3534%)
Q30 bases: 259603287(92.15%)

Read1 after filtering:
total reads: 1955705
total bases: 281477167
Q20 bases: 274168515(97.4035%)
Q30 bases: 259546963(92.2089%)

Filtering result:
reads passed filter: 1955705
reads failed due to low quality: 263
reads failed due to too many N: 409
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 93.1268%

JSON report: SewardAK_312.json
HTML report: SewardAK_312.html

fastp -i SewardAK_312.F.fq.gz -o SewardAK_312.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_312.json -h SewardAK_312.html 
fastp v0.20.0, time used: 27 seconds