WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1837184
total bases: 264554496
Q20 bases: 257614849(97.3769%)
Q30 bases: 243910447(92.1967%)

Read1 after filtering:
total reads: 1836441
total bases: 264164337
Q20 bases: 257487080(97.4723%)
Q30 bases: 243849306(92.3097%)

Filtering result:
reads passed filter: 1836441
reads failed due to low quality: 308
reads failed due to too many N: 435
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 92.7372%

JSON report: SewardAK_311.json
HTML report: SewardAK_311.html

fastp -i SewardAK_311.F.fq.gz -o SewardAK_311.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_311.json -h SewardAK_311.html 
fastp v0.20.0, time used: 25 seconds