WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1885261
total bases: 271477584
Q20 bases: 264222425(97.3275%)
Q30 bases: 250093684(92.1231%)

Read1 after filtering:
total reads: 1884653
total bases: 271231806
Q20 bases: 264130996(97.382%)
Q30 bases: 250040729(92.1871%)

Filtering result:
reads passed filter: 1884653
reads failed due to low quality: 218
reads failed due to too many N: 390
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 92.7728%

JSON report: SewardAK_310.json
HTML report: SewardAK_310.html

fastp -i SewardAK_310.F.fq.gz -o SewardAK_310.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_310.json -h SewardAK_310.html 
fastp v0.20.0, time used: 26 seconds