WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1662117
total bases: 239344848
Q20 bases: 232777536(97.2561%)
Q30 bases: 220232394(92.0147%)

Read1 after filtering:
total reads: 1661537
total bases: 239120395
Q20 bases: 232694444(97.3127%)
Q30 bases: 220184908(92.0812%)

Filtering result:
reads passed filter: 1661537
reads failed due to low quality: 239
reads failed due to too many N: 341
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 92.278%

JSON report: SewardAK_309.json
HTML report: SewardAK_309.html

fastp -i SewardAK_309.F.fq.gz -o SewardAK_309.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_309.json -h SewardAK_309.html 
fastp v0.20.0, time used: 24 seconds