WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2542559
total bases: 366128496
Q20 bases: 356464385(97.3605%)
Q30 bases: 337746568(92.2481%)

Read1 after filtering:
total reads: 2541675
total bases: 365670777
Q20 bases: 356314312(97.4413%)
Q30 bases: 337672472(92.3433%)

Filtering result:
reads passed filter: 2541675
reads failed due to low quality: 351
reads failed due to too many N: 533
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 92.5419%

JSON report: SewardAK_306.json
HTML report: SewardAK_306.html

fastp -i SewardAK_306.F.fq.gz -o SewardAK_306.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_306.json -h SewardAK_306.html 
fastp v0.20.0, time used: 30 seconds