WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2749001
total bases: 395856144
Q20 bases: 385230359(97.3157%)
Q30 bases: 364845559(92.1662%)

Read1 after filtering:
total reads: 2747931
total bases: 395261677
Q20 bases: 385041951(97.4144%)
Q30 bases: 364759894(92.2831%)

Filtering result:
reads passed filter: 2747931
reads failed due to low quality: 464
reads failed due to too many N: 606
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 91.67%

JSON report: SewardAK_305.json
HTML report: SewardAK_305.html

fastp -i SewardAK_305.F.fq.gz -o SewardAK_305.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_305.json -h SewardAK_305.html 
fastp v0.20.0, time used: 33 seconds