WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2251157
total bases: 324166608
Q20 bases: 315337831(97.2765%)
Q30 bases: 298244236(92.0034%)

Read1 after filtering:
total reads: 2250186
total bases: 323814266
Q20 bases: 315206321(97.3417%)
Q30 bases: 298170414(92.0807%)

Filtering result:
reads passed filter: 2250186
reads failed due to low quality: 470
reads failed due to too many N: 501
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 91.3866%

JSON report: SewardAK_304.json
HTML report: SewardAK_304.html

fastp -i SewardAK_304.F.fq.gz -o SewardAK_304.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_304.json -h SewardAK_304.html 
fastp v0.20.0, time used: 30 seconds